The We and the I
(2012, Michael Brodie, Teresa Lynn)
Directed by Michel Gondry, this lively movie about the lives of a group of teenagers opens with this charming model New York City Transit Orion VII Second Generation Hybrid Low Floor, which carries fleet number 4601 on the roof. It meets a sad end when run over and crushed by the real 4601, a 2010 Orion VII Next Generation Hybrid Low Floor.
Actually much of the movie takes place aboard the full size 4601:
There's also a glimpse of an older RTS: