Dirty Harry
(1971, Clint Eastwood)
Harry Callahan (Eastwood) boards PCC streetcar 1169 of the San Francisco Municipal Railway while chasing a villain. The car is boarded in one of the stations in the long tunnel section in the SF suburbs. (thanks Alan Sinclair)
Later in the movie a San Francisco Ford B series school bus (carrying the fleetname Dechsel Charter Service) is hijacked. Callahan jumps on the roof: